FastQCFastQC Report
Sat 25 May 2024
EGAF00001146183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001146183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85438566
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA2116200.24768674137157215TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1018820.11924591524628352TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)