FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001146588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001146588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57208699
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3070710.5367557825427913TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA612020.10698023389764554TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)