FastQCFastQC Report
Wed 15 May 2024
EGAF00001146606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001146606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58548376
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1533200.26186892015587243TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA928970.15866708241403657TruSeq Adapter, Index 19 (97% over 40bp)
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA831560.1420295585995417No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT820930.140213965968928No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG590750.10089946815945842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)