FastQCFastQC Report
Wed 8 Feb 2023
EGAF00001147248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001147248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225096444
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT57157862.5392609045392116Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG2488060.11053306555122656Illumina Paired End Adapter 2 (96% over 28bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2433540.10811099263744921No Hit
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG2364060.10502431571064713Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)