FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001147254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001147254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208379243
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT63739073.0588013029685492Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG9415660.4518521069778529Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGA6242900.29959317972951843Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG3049600.14634854969695804Illumina Paired End Adapter 2 (96% over 28bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGC2556750.12269696171225654Illumina Single End PCR Primer 1 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)