FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001147256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001147256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207933197
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT63595683.058466897904715Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG9182710.44161827608508325Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGA6238760.3000367468980915Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG3143010.15115479612425717Illumina Paired End Adapter 2 (96% over 28bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGC2514890.12094701742117685Illumina Single End PCR Primer 1 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)