FastQCFastQC Report
Mon 23 Jan 2023
EGAF00001147285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001147285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207842900
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG85319094.104979770778796Illumina Paired End PCR Primer 2 (100% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG11151800.536549480400822Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG9181230.44173892877745646Illumina Paired End PCR Primer 2 (97% over 36bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC2660410.12800100460492037Illumina Paired End PCR Primer 2 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)