FastQCFastQC Report
Mon 26 Dec 2022
EGAF00001147286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001147286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207842900
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT56049082.696704097181092Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG9471160.45568840696506835Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGA6039890.29059881285336187Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGC2437180.11726068102398494Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG2164770.1041541471948284Illumina Paired End Adapter 2 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)