FastQCFastQC Report
Wed 8 Feb 2023
EGAF00001147366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001147366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227340112
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT67229132.9572049300301213Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG3351870.14743856552687895Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG2607860.11471182876869525Illumina Paired End Adapter 2 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)