FastQCFastQC Report
Sun 19 May 2024
EGAF00001150223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001150223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151620412
Total Bases15.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4675080.3083410695388428Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG2974730.19619587895592844Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG1961240.1293519766982298Illumina Single End PCR Primer 1 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)