FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001167813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001167813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64424191
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3909190.6067891485047907TruSeq Adapter, Index 9 (100% over 51bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAG1193610.18527357215863216No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGT1183600.18371980798330861No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGT1055940.1639042700590528No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGG1045820.16233343155213234No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC928960.14419428254830552No Hit
CCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGC885520.13745147377946895No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATTG826090.12822667808121954No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAA797970.12386185804025074No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTC729580.1132462804228306No Hit
TGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT688300.10683874943807987No Hit
GTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGG651120.10106762535830678No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGA649240.10077580950919507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)