FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001170334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001170334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12678285
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT3722872.936414507167176TruSeq Adapter, Index 21 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC145080.11443188096812779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)