FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001170582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001170582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659956
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1133646.829337645094208Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC101810.6133295099388177Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT42020.25313923983527276No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT40760.24554867719385332No Hit
TGTGTCGCTGGTGCGGTCTCACGGCATTTCTTGGCGCCACAAGATCGGAA23870.14379899226244552No Hit
TGTGTCGCTGGTGCGGTCTCAGAGACCATCCTGGCTGACACAAGATCGGA22210.13379872719517868No Hit
CGTGTCGCTGGTGCGGTCTCACCGCACGGCACCTCGCCACGAGATCGGAA21200.1277142285699139No Hit
TGTGTCGCTGGTGCGGTCTCAGTTAAGCAAGGACCAGTGTCACAAGATCG20380.12277433859692666No Hit
TGTGTCGCTGGTGCGGTCTCACATTGGCTCCAGGCGTCACAAGATCGGAA18810.11331625657547549No Hit
CGTGTCGCTGGTGCGGTCTCACAATGAGCAGCCCCGTCACGAGATCGGAA18280.11012340086122764No Hit
TGTGTCGCTGGTGCGGTCTCATCTTGGTGCCCTGCGCCACAAGATCGGAA16640.10024362091525317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)