FastQCFastQC Report
Sat 11 May 2024
EGAF00001170597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001170597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15391043
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4787893.1108288112767926TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA3481662.262133891770688TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT329070.21380617285001413TruSeq Adapter, Index 15 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT233590.15177009121474094TruSeq Adapter, Index 15 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC215320.139899550667229TruSeq Adapter, Index 15 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGA194110.12611880819253118TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT170980.11109058690824267TruSeq Adapter, Index 15 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT160170.10406702131882811TruSeq Adapter, Index 15 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)