FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001172363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001172363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463097
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC180700.7336292480564103Illumina Single End PCR Primer 1 (100% over 50bp)
CTAAAGGTAGTGTTGTTAAAAAGGTCTTCCCCCAAATGCTCCTTGCTTAA29890.12135129067186554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)