FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501536
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA1339318.919599663278136TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATATCGTA59510.39632749397949835TruSeq Adapter, Index 7 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGCCGTC29340.19539991049165656TruSeq Adapter, Index 7 (96% over 29bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT28690.19107100995247533No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT27890.18574313236579076No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA18400.12254118449374507No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGC17750.11821228395456386TruSeq Adapter, Index 7 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)