FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608594
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTA1205857.496298009317454TruSeq Adapter, Index 25 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATATCGTA96560.6002757687769568TruSeq Adapter, Index 25 (97% over 37bp)
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT42960.26706552430258973No Hit
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT41580.2584866038291825No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA29860.18562794589560822No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA21030.13073528808387946No Hit
TGTGTCAGCCAGGATGGTCTCTGAGACCGCACCAGCGACACAAGATCGGA20680.12855947492033418No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA20560.12781348183569005No Hit
TGTGACACTGGTCCTTGCTTAACTGAGACCGCACCAGCGACACAAGATCG18330.1139504436793871No Hit
TGTGACGCCTGGAGCCAATGTGAGACCGCACCAGCGACACAAGATCGGAA18190.11308011841396896No Hit
CGTGACGGGGCTGCTCATTGTGAGACCGCACCAGCGACACGAGATCGGAA16470.10238755086740346No Hit
TGTGGCAGAGGAGTGAGTTTTGTGAGACCGCACCAGCGACACAAGATCGG16370.10176588996353338No Hit
TGTGGCGTCATGGGCCTCAGTGAGACCGCACCAGCGACACAAGATCGGAA16190.10064690033656722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)