FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608594
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1267907.882038600168843Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC104850.651811457707787Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT44000.2735307977028386No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT43470.2702359949123272No Hit
TGTGTCGCTGGTGCGGTCTCACGGCATTTCTTGGCGCCACAAGATCGGAA27000.16784844404492372No Hit
TGTGTCGCTGGTGCGGTCTCAGAGACCATCCTGGCTGACACAAGATCGGA21320.13253810470510272No Hit
CGTGTCGCTGGTGCGGTCTCACCGCACGGCACCTCGCCACGAGATCGGAA20080.12482950949711363No Hit
TGTGTCGCTGGTGCGGTCTCAGTTAAGCAAGGACCAGTGTCACAAGATCG18490.11494510112557924No Hit
TGTGTCGCTGGTGCGGTCTCACATTGGCTCCAGGCGTCACAAGATCGGAA18000.1118989626966158No Hit
CGTGTCGCTGGTGCGGTCTCACAATGAGCAGCCCCGTCACGAGATCGGAA16790.10437686575978775No Hit
TGTGTCGCTGGTGCGGTCTCACAAAACTCACTCCTCTGCCACAAGATCGG16420.10207672041546842No Hit
CGTGTCGCTGGTGCGGTCTCAGCCACGATGGCAGCAGCACGAGATCGGAA16360.10170372387314636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)