FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679817
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTA934915.565546723244258TruSeq Adapter, Index 2 (97% over 38bp)
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT22050.1312642984325078No Hit
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT21940.13060946519769714No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA17800.10596392345118547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)