FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511433
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA1038246.869242632653911TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATATCGTA126400.8362924456459533TruSeq Adapter, Index 5 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTATGCCGTC39250.25968732983863657TruSeq Adapter, Index 5 (96% over 30bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT21030.13913947889188605No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT20840.13788239372833597No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTATGC16490.10910175972074183TruSeq Adapter, Index 5 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)