FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005040
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTA46449623.166420620037506TruSeq Adapter, Index 13 (97% over 38bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGCCGTC268501.3391254039819656TruSeq Adapter, Index 13 (96% over 32bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGC162600.8109563898974584TruSeq Adapter, Index 13 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATATCGTA128020.6384910026732634TruSeq Adapter, Index 13 (97% over 38bp)
ACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGC57070.2846327255316602TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATG50320.2509675617444041TruSeq Adapter, Index 13 (97% over 37bp)
TCGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGCC47680.23780074212983285TruSeq Adapter, Index 13 (96% over 33bp)
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT45900.22892311375334157No Hit
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT45420.22652914655069226No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA26570.13251605952998444No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA26280.13106970434505047No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA26030.12982284642700395No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATTTCGTA25040.12488528907153973TruSeq Adapter, Index 13 (97% over 38bp)
AGCGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGC23980.11959861149902246TruSeq Adapter, Index 13 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTAATCTCGTA23270.11605753501177034TruSeq Adapter, Index 13 (97% over 38bp)
ATGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGC23000.11471092846028008TruSeq Adapter, Index 13 (97% over 34bp)
TGTGACACTGGTCCTTGCTTAACTGAGACCGCACCAGCGACACAAGATCG22820.1138131907592866No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGCCGTCT20590.10269121813031162TruSeq Adapter, Index 13 (96% over 31bp)
TGTGACGCCTGGAGCCAATGTGAGACCGCACCAGCGACACAAGATCGGAA20370.10159398316243068No Hit
GTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGC20150.10049674819454972TruSeq Adapter, Index 13 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)