FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005040
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC41203220.549814467541793Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC836354.171238479032837Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT47410.23645413557834258No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT45680.22782587878546062No Hit
CGTGTCGCTGGTGCGGTCTCACCGCACGGCACCTCGCCACGAGATCGGAA24080.12009735466624108No Hit
TGTGTCGCTGGTGCGGTCTCAGTTAAGCAAGGACCAGTGTCACAAGATCG21990.10967362247137215No Hit
TGTGTCGCTGGTGCGGTCTCACGGCATTTCTTGGCGCCACAAGATCGGAA21870.10907513067070981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)