FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936242
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTA1507347.784873998188243TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATATCGTA136990.7075045371394691TruSeq Adapter, Index 22 (97% over 37bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT34570.17854173187029307No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT34050.17585611715890884No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA24500.12653377005560257No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA21790.11253758569435018No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA21020.10856081006403126No Hit
TGTGACACTGGTCCTTGCTTAACTGAGACCGCACCAGCGACACAAGATCG19380.10009079443581949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)