FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998853
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTA323171.616777221736666TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATATCGTA32670.16344373498201217TruSeq Adapter, Index 14 (97% over 38bp)
AGGTGGATAAGAAAACAATGAATTACTCCATCAAAAGCAAAAGCACAAGC21550.1078118300845535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)