FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431071
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTA591624.134106553762881TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATATCGTA34150.23863246477638078TruSeq Adapter, Index 23 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATGCCGTC15390.10754183405295753TruSeq Adapter, Index 23 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)