FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299801
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA1657757.208232364452402TruSeq Adapter, Index 5 (97% over 37bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT40160.1746238044074248No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT38180.16601436385148108No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGC29030.12622831279749858TruSeq Adapter, Index 5 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATATCGTA26010.11309674184853385TruSeq Adapter, Index 5 (97% over 37bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA24480.10644399232803187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)