FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806130
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTA1112126.157474821856677TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATATCGTA34940.19345229856101168TruSeq Adapter, Index 7 (97% over 36bp)
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT25820.14295759441457703No Hit
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT25620.14185025441136576No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCACAATCTCGTA23100.12789777037090355TruSeq Adapter, Index 7 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTATGC20050.11101083532193143TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)