FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812247
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTA448562.475159291200372TruSeq Adapter, Index 15 (97% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGC39370.2172441173857647TruSeq Adapter, Index 15 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCCGTC38880.2145402916931301TruSeq Adapter, Index 15 (96% over 31bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTA33110.18270136465945316TruSeq Adapter, Index 12 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)