FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423585
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTA24587210.144971189374418TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATATCGTA50300.20754378327972814TruSeq Adapter, Index 8 (97% over 37bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT36500.15060334174373913No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT35960.14837523750972215No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA25760.10628882420051287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)