FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808287
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1680029.290671226414833Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT65900.3644333006873356No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT62480.34552037370174093No Hit
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC48660.2690944523739871Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCACGGCATTTCTTGGCGCCACAAGATCGGAA37620.20804219684154118No Hit
TGTGTCGCTGGTGCGGTCTCAGAGACCATCCTGGCTGACACAAGATCGGA34560.19112010427548282No Hit
TGTGTCGCTGGTGCGGTCTCAGTTAAGCAAGGACCAGTGTCACAAGATCG32020.1770736614265324No Hit
CGTGTCGCTGGTGCGGTCTCACCGCACGGCACCTCGCCACGAGATCGGAA30060.16623467403127934No Hit
TGTGTCGCTGGTGCGGTCTCACATTGGCTCCAGGCGTCACAAGATCGGAA29950.1656263635141988No Hit
CGTGTCGCTGGTGCGGTCTCACAATGAGCAGCCCCGTCACGAGATCGGAA26050.14405899063588912No Hit
CGTGTCGCTGGTGCGGTCTCAGCCACGATGGCAGCAGCACGAGATCGGAA25050.1385288950260661No Hit
TGTGTCGCTGGTGCGGTCTCATCTTGGTGCCCTGCGCCACAAGATCGGAA24860.13747817686019975No Hit
CGTGTCGCTGGTGCGGTCTCAGACCCAGTCCCACTGACACGAGATCGGAA21600.11945006517217675No Hit
TGTGTCGCTGGTGCGGTCTCACTGAGGCCCATGACGCCACAAGATCGGAA21200.11723802692824757No Hit
TGTGTCGCTGGTGCGGTCTCAACTCAGCTTCCTGCCCCACAAGATCGGAA20800.11502598868431836No Hit
CGTGTCGCTGGTGCGGTCTCAGTCCTAGGTTCCTGCAACACGAGATCGGA20640.11414117338674667No Hit
TGTGTCGCTGGTGCGGTCTCATAAATAAACTACGCACATCCTCACAAGAT20350.11253744565989801No Hit
CGTGTCGCTGGTGCGGTCTCATCGAGTCTACTCGCCACACGAGATCGGAA20130.11132082462573696No Hit
TGTGTCGCTGGTGCGGTCTCAGCCTGACTTACACCTCATCATCACAAGAT19340.10695204909397678No Hit
TGTGTCGCTGGTGCGGTCTCACAAAACTCACTCCTCTGCCACAAGATCGG18770.10379989459637767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)