FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066088
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTA1409326.821200258653068TruSeq Adapter, Index 11 (97% over 38bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT40140.19428020490898743No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT39580.19156976856745694No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATATCGTA35930.17390353169855302TruSeq Adapter, Index 11 (97% over 38bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA29540.14297551701573213No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA23120.11190230038604358No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA22330.1080786491185274No Hit
TGTGTCAGCCAGGATGGTCTCTGAGACCGCACCAGCGACACAAGATCGGA21030.10178656475426022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)