FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175195
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTA1566577.201974995345245TruSeq Adapter, Index 20 (97% over 38bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT48490.22292254257664257No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT46750.21492325975372323No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATATCGTA40270.18513282717181678TruSeq Adapter, Index 20 (97% over 38bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA32610.149917593595057No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA25490.1171848960667894No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA24610.11313928176554286No Hit
TGTGTCAGCCAGGATGGTCTCTGAGACCGCACCAGCGACACAAGATCGGA24220.11134633906385405No Hit
TGTGACACTGGTCCTTGCTTAACTGAGACCGCACCAGCGACACAAGATCG23280.10702488742388615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)