FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137745
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTA1355806.342197034725844TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATATCGTA49240.23033617199432113TruSeq Adapter, Index 14 (97% over 38bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT32500.15202935803849382No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT32210.15067278838215034No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTATGC30470.14253337044408945TruSeq Adapter, Index 14 (97% over 36bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA21530.10071360241750069No Hit

[FAIL]Adapter Content

Adapter graph