FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357952
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTA964987.106142190592893TruSeq Adapter, Index 1 (97% over 37bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT21150.1557492459232727No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT20400.15022622301819208No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATATCGTA15710.11568891978508815TruSeq Adapter, Index 1 (97% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATGC14560.10722028466396455TruSeq Adapter, Index 1 (97% over 35bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA14140.10412739183711943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)