FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770624
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTA1628129.195176389792525TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATATCGTA125100.7065305790500976TruSeq Adapter, Index 23 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTC120700.6816805826646425TruSeq Adapter, Index 23 (96% over 31bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGC71670.4047725547603557TruSeq Adapter, Index 23 (97% over 35bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTA28740.16231565820863153TruSeq Adapter, Index 23 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATG24630.139103502494036TruSeq Adapter, Index 23 (97% over 36bp)
CACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTTCTGCT17930.10126373527072942TruSeq Adapter, Index 8 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)