FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001179194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686378
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA1575359.341618545782737TruSeq Adapter, Index 1 (97% over 36bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT39460.2339926161275823No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT38730.22966381202790834No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA24680.14634915778075852No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA20020.11871596996640137No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA19740.11705560675008807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)