FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091695
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTA1536707.346673391675172TruSeq Adapter, Index 22 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTATGCCGTC48060.22976581193720882TruSeq Adapter, Index 22 (96% over 31bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTATGC32490.15532857323845015TruSeq Adapter, Index 22 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATATCGTA32360.154707067713027TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)