FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961070
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1411947.199844982586037Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC235791.202353817048856Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT25890.13201976472028026No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT25850.13181579443875027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)