FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154439
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTA1119705.19717661999249TruSeq Adapter, Index 5 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATATCGTA46710.2168081806911219TruSeq Adapter, Index 5 (97% over 39bp)
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT44170.20501856863898213No Hit
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT43170.20037698909089557No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGGA40340.18724131896981072TruSeq Adapter, Index 5 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCCCGTA30850.14319272905846953TruSeq Adapter, Index 5 (97% over 39bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA30780.14286781849010347No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA26830.12453357927516165No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA24800.115111172792546No Hit
TGTGACACTGGTCCTTGCTTAACTGAGACCGCACCAGCGACACAAGATCG22510.10448195562742783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)