FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154439
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1308986.0757347968543085Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC105430.4893617317547631Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT44210.20520423182090558No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT43060.19986641534060606No Hit
TGTGTCGCTGGTGCGGTCTCACGGCATTTCTTGGCGCCACAAGATCGGAA24000.11139790915407678No Hit
CGTGTCGCTGGTGCGGTCTCACCGCACGGCACCTCGCCACGAGATCGGAA23720.11009826688061254No Hit
TGTGTCGCTGGTGCGGTCTCAGTTAAGCAAGGACCAGTGTCACAAGATCG21580.10016528664770737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)