FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095333
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA25038511.949651916902946TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATATCGTA467032.2289058588778015TruSeq Adapter, Index 7 (97% over 39bp)
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT72890.34786833405477796No Hit
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT69970.3339326016437483No Hit
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA49920.23824375409541107No Hit
TGTGGCGCAGGGCACCAAGATGAGACCGCACCAGCGACACAAGATCGGAA36350.17348077847292054No Hit
CGTGGCGAGGTGCCGTGCGGTGAGACCGCACCAGCGACACGAGATCGGAA35010.16708561359936583No Hit
TGTGACACTGGTCCTTGCTTAACTGAGACCGCACCAGCGACACAAGATCG33970.16212220205571143No Hit
TGTGACGCCTGGAGCCAATGTGAGACCGCACCAGCGACACAAGATCGGAA33150.15820874295398393No Hit
TGTGTCAGCCAGGATGGTCTCTGAGACCGCACCAGCGACACAAGATCGGA32700.15606111295913347No Hit
CGTGACGGGGCTGCTCATTGTGAGACCGCACCAGCGACACGAGATCGGAA29220.13945277433228992No Hit
TGTGTCGCTGGTGCGGTCTCAAGTCAGGGGCACCCAACACAAGATCGGAA29190.13930959899929987No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTATGC28410.13558704034155908TruSeq Adapter, Index 7 (97% over 37bp)
CGTGCTGCTGCCATCGTGGCTGAGACCGCACCAGCGACACGAGATCGGAA26340.12570794236524696No Hit
TGTGGCGTCATGGGCCTCAGTGAGACCGCACCAGCGACACAAGATCGGAA26200.1250397908112935No Hit
CGTGTGGCGAGTAGACTCGATGAGACCGCACCAGCGACACGAGATCGGAA25390.12117405682056265No Hit
TGTGGCAGAGGAGTGAGTTTTGTGAGACCGCACCAGCGACACAAGATCGG24790.11831055016076203No Hit
TGTGGGGCAGGAAGCTGAGTTGAGACCGCACCAGCGACACAAGATCGGAA24550.1171651474968418No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATTTCGTA24430.11659244616488167TruSeq Adapter, Index 7 (97% over 39bp)
TGTGATGATGAGGTGTAAGTCAGGCTGAGACCGCACCAGCGACACAAGAT24410.11649699594288832No Hit
TGTGAGGATGTGCGTAGTTTATTTATGAGACCGCACCAGCGACACAAGAT23770.113442588839101No Hit
CGTGTTGCAGGAACCTAGGACTGAGACCGCACCAGCGACACGAGATCGGA23480.11205856062019737No Hit
CGTGTCAGTGGGACTGGGTCTGAGACCGCACCAGCGACACGAGATCGGAA23220.11081770773428376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)