FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095333
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC28376813.54285929730501Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC220321.0514796454787856Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCCCCCCCTGCCACAAGAT73390.35025458960461175No Hit
TGTGTCGCTGGTGCGGTCTCAAAAGTTCTGTCCTCCCCCTGCCACAAGAT71940.3433344485100936No Hit
TGTGTCGCTGGTGCGGTCTCACGGCATTTCTTGGCGCCACAAGATCGGAA41170.1964842819733188No Hit
TGTGTCGCTGGTGCGGTCTCAGTTAAGCAAGGACCAGTGTCACAAGATCG33300.15892461961893406No Hit
TGTGTCGCTGGTGCGGTCTCAGAGACCATCCTGGCTGACACAAGATCGGA32710.15610883807013015No Hit
CGTGTCGCTGGTGCGGTCTCACCGCACGGCACCTCGCCACGAGATCGGAA31970.15257717985637603No Hit
TGTGTCGCTGGTGCGGTCTCACATTGGCTCCAGGCGTCACAAGATCGGAA31660.15109770141547907No Hit
TGTGTTGGGTGCCCCTGACTTGAGACCGCACCAGCGACACAAGATCGGAA29280.13973912499826996No Hit
CGTGTCGCTGGTGCGGTCTCACAATGAGCAGCCCCGTCACGAGATCGGAA28320.135157514342589No Hit
CGTGTCGCTGGTGCGGTCTCAGCCACGATGGCAGCAGCACGAGATCGGAA26050.12432391414634333No Hit
CGTGTCGCTGGTGCGGTCTCATCGAGTCTACTCGCCACACGAGATCGGAA24710.11792874927278862No Hit
TGTGTCGCTGGTGCGGTCTCACTGAGGCCCATGACGCCACAAGATCGGAA24500.1169265219418584No Hit
TGTGTCGCTGGTGCGGTCTCACAAAACTCACTCCTCTGCCACAAGATCGG24160.11530386816797139No Hit
TGTGTCGCTGGTGCGGTCTCAGCCTGACTTACACCTCATCATCACAAGAT23950.11430164083704118No Hit
TGTGTCGCTGGTGCGGTCTCAACTCAGCTTCCTGCCCCACAAGATCGGAA23460.111963110398204No Hit
TGTGTCGCTGGTGCGGTCTCATAAATAAACTACGCACATCCTCACAAGAT23350.11143813417724055No Hit
CGTGTCGCTGGTGCGGTCTCAGACCCAGTCCCACTGACACGAGATCGGAA22650.10809737640747318No Hit
CGTGTCGCTGGTGCGGTCTCAGTCCTAGGTTCCTGCAACACGAGATCGGA22630.10800192618547982No Hit
TGTGTCGCTGGTGCGGTCTCATCTTGGTGCCCTGCGCCACAAGATCGGAA21970.10485206885969915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)