FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001179212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001179212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198102
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTA2074989.439871307154991TruSeq Adapter, Index 9 (97% over 38bp)
TGTGGCAGGGGGAGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT35530.16163945076252148No Hit
TGTGGCAGGGGGGGGACAGAACTTTTGAGACCGCACCAGCGACACAAGAT34000.15467890025121672No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGAATCTCGTA32160.14630804211997442TruSeq Adapter, Index 9 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTATGC28420.12929336309234057TruSeq Adapter, Index 9 (97% over 36bp)
TGTGGCGCCAAGAAATGCCGTGAGACCGCACCAGCGACACAAGATCGGAA24450.11123232679830145No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGCATCTCGTA23070.10495418319986971TruSeq Adapter, Index 9 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)