FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001207763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001207763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401115
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1811643.3541963094657303TruSeq Adapter, Index 12 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165590.3065848440553478No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC76670.1419521709869166TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)