FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001207769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001207769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663667
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2655819.970503069640461TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2343808.79914794154074TruSeq Adapter, Index 2 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAACTCGTATGCC383811.4409083417709496TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTACCTCGTATGC113800.42723058099980216TruSeq Adapter, Index 2 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTACCTCGTATGCC86960.3264672348307803TruSeq Adapter, Index 2 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82460.30957323118843305No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAACTCGTATGC79560.29868598439669825TruSeq Adapter, Index 2 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGCC57080.21429105064559498TruSeq Adapter, Index 2 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGCC52560.19732196254261514TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC42810.16071828798419624TruSeq Adapter, Index 2 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGTATCTCGTATGCC39040.1465648671549409TruSeq Adapter, Index 2 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCCCGTATGCC29190.10958577029335874TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)