FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001211446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12022873
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG2069071.7209447359212728TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1844611.5342505905202526TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCCATTATCTCGTATG303160.2521527092567642TruSeq Adapter, Index 11 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAACTCGTATG243300.20236427682468242TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)