FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001211455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15829626
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1359650.8589274313871976TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG692990.43778039986541695TruSeq Adapter, Index 27 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAACTCGTATG219270.1385187495901672TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph