FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001211456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16949364
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG398290.23498816828761243TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTATG186320.10992742854540147TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT172960.10204512688499698TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph