FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001211460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640749
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG1658254.554694652116913TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAACTCGTATG181400.4982491240126688TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCCATTATCTCGTATG150400.41310180954523373TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT126270.34682423863880757TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)