FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001211464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001211464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7526133
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT618750.8221353515809514TruSeq Adapter, Index 13 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATG259150.3443335375550764TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG117050.15552475620614198TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)